The Effectiveness Of Moringa Extract Cream On Collagen Fiber Density In The Wound Healing Process After Incision In White Rats (Rattus Norvegicus)

Abstract

Moringa (Moringa oleifera) leaf extract cream is believed to accelerate wound healing by enhancing collagen fiber density and compactness in skin tissue. This experimental study aimed to evaluate the effectiveness of Moringa oleifera leaf extract cream on collagen fiber density during the healing of incision wounds in white rats (Rattus norvegicus). Twenty-four male C57BL/6 strain rats were divided into four groups: control (placebo) and treatment groups receiving 5%, 10%, and 15% moringa extract cream. The cream was applied twice daily for 14 days. Wound tissues were then examined histopathologically using Masson’s Trichrome staining to assess collagen density. Macroscopic observations showed that the treatment groups, particularly the 15% concentration, exhibited faster wound closure compared to the control. Histologically, the treatment groups demonstrated higher collagen deposition and denser fiber organization. However, one-way ANOVA analysis (p = 0.791) indicated no statistically significant differences among groups. Despite the lack of significance, an increasing trend in collagen density was evident in the 10% and 15% treatment groups. These findings suggest that Moringa oleifera extract cream may promote wound healing and improve collagen formation, though further studies with larger samples and longer observation periods are needed to confirm its efficacy.