The Effect Of Nanoemulsion Preparation Of Bay Leaf Extract ( Syzygium Polyanthum ) On Reducing Regenerating Islet-Derived Protein 3 Beta (Reg3β) And C-Reactive Protein (Crp) Levels In Wistar Rats With Diabetes Mellitus

Abstract

This study aims to evaluate the effect of bay leaf ( Syzygium polyanthum ) extract nanoemulsion preparations on reducing regenerating islet-derived protein 3 beta (REG3β) and C-reactive protein (CRP) levels in Wistar rats with diabetes mellitus. The method used includes the preparation of nanoemulsion preparations and measurement of REG3β and CRP levels as indicators of inflammation and pancreatic damage. Rats were divided into control and treatment groups, where the treatment group received bay leaf extract nanoemulsion preparations. The results showed that treatment with nanoemulsion significantly reduced REG3β and CRP levels compared to the control group. Nanoemulsion extract Bay leaves ( Syzygium Polyanthum) contain secondary metabolites in the form of saponins, tannins, flavonoids, and triterpenoids which help repair pancreatic cells that experience inflammation due to diabetes mellitus. Administration of nanoemulsion extract Bay leaves ( Syzygium polyanthum) at a concentration of 3.5% were effective in improving liver function in Wistar white rats ( Rattus norvegicus ) with diabetes mellitus. This improvement can be seen through CRP levels and liver histology structure which improved and resembled the control group . The results of histopathological observations of pancreatic tissue in treatment group 3 were as follows: nanoemulsion extract Bay leaves ( Syzygium Polyanthum) with a concentration of 3.5% experienced the most significant improvement and approached the control group compared to the other groups. This finding indicates that bay leaf extract has potential as an anti-inflammatory agent and pancreatic protector in diabetes mellitus.