Gc-Ms Instrument Analysis of Turmeric Rhizome Extract Nanoemulsion Preparation (Curcuma Domestica Val.) on Amylase, Lipase Levels and Pancreatic Beta Cell Damage Scoring in Male Rats with Diabetes Mellitus and Histopathological Images of the Pancreas

Abstract

Study This aim For analyze stock nanoemulsi extract rhizome turmeric ( Curcuma domestica Val .) uses GC-MS instruments , as well as the impact to level amylase , lipase, and damage scoring pancreatic beta cells on mouse males who suffer from diabetes mellitus . In addition that , research This Also evaluate description histopathology pancreas.
Results study show that The results of GC-MS (Gas Chromatography-Mass Spectrometry) analysis for turmeric compound content usually include a number of chemical compounds that have various health benefits. Here are some of the main compounds often found in turmeric: Curcumin (30%): The main active compound that gives turmeric its yellow color and has anti-inflammatory and antioxidant properties, demethoxycurcumin (15.3%): A variant of curcumin with anti-inflammatory properties, bisdemethoxycurcumin (8.2%): Other compounds that play a role in the biological activity of turmeric, turmerone (5.7%): Compounds that contribute to aroma and have potential therapeutic effects, zingiberene (41%), beta-turmerone (2.8%) and volatile compounds (33.4): Such as zingiberene and beta-turmerone, which also have health benefits. Nanoemulsion of turmeric rhizome extract ( Curcuma Domestica val.) was proven to be effective in reducing amylase and lipase levels in alloxan-induced male white rats (Rattus norvegicus). The treatment group given nanoemulsion of turmeric rhizome extract ( Curcuma Domestica val.) at a dose of 180 mg/kgbb showed the most optimal results, with a significant decrease in amylase and lipase levels, approaching the values shown by the control group. In histopathological examination of the pancreas, the group given nanoemulsion of turmeric rhizome extract ( Curcuma Domestica val.) at a dose of 180 mg/kgbb also showed minimal tissue damage, marked by reduced β cell necrosis, reduced vacuosis, and increased islet density. Score 1 because the number of degenerative cells with a degree of damage (<1-25%) of the entire LP (Field of View), the number of necrotic cells <25% of the entire LP. and the number of inflammatory cells <10 in the entire space.

Keywords:    Nanoemulsi Turmeric , GC-MS, Amylase , Lipase, Pancreas.