The Effectiveness of Kaffir Lime Leaf Extract (Citrus hystrix DC) on Incised Wound in Wistar Rats (Rattus novergicus)

Abstract

Incised wounds are injuries caused by cuts from sharp instruments, characterized by open wounds, pain, and a wound length greater than its depth. The extract of kaffir lime leaf (Citrus hystrix DC) is believed to have antibacterial and anti-inflammatory properties that can accelerate the wound healing process. This study aims to determine the effectiveness of kaffir lime leaf extract on the healing of incised wounds in Wistar rats. This research used a true experimental method with a control group design. A total of 25 rats were divided into five groups: a negative control group (P0) treated with 0.9% NaCl, a positive control group (P4) treated with 0.1% gentamicin, and three treatment groups (P1, P2, P3) treated with kaffir lime leaf extract at concentrations of 10%, 20%, and 30%. Observations were carried out for 14 days. The results showed macroscopic changes, particularly in the average wound length and wound condition characterized by erythema, redness, dry open wounds, and dry closed wounds. Histopathological observations revealed the highest number of fibroblasts and collagen fibers in group P1. ANOVA test showed no significant difference between groups (p > 0.05), although the 10% extract proved to be more effective in healing incised wounds.