Evaluation of the Antibacterial Potential of Jatropha multifida Leaf Extract using Microwave-Assisted Extraction

Abstract

Oral diseases, such as dental caries and oral candidiasis, are often caused by a microbial imbalance. Conventional antifungal and antibacterial treatments, including Nystatin and Povidone-Iodine, face challenges like microbial resistance and side effects. Given Indonesia's rich biodiversity and the public's growing interest in herbal medicine. This study aims to explore the antimicrobial potential of Jatropha multifida leaf extract as a base for a new mouthwash formulation. Utilizing a post-test only control group experimental design, the study first extracted Jatropha multifida leaves with Microwave-Assisted Extraction. The agar disk diffusion method was then employed to screen various extract concentrations against Staphylococcus aureus to identify the most effective one. The collected data on microbial inhibition zones will be statistically analyzed using a One-Way ANOVA and the Dunnet Post Hoc test. We anticipate that the Jatropha multifida will exhibit significant antimicrobial activity, suggesting its potential as a natural and effective alternative to conventional antiseptics. This research provides a foundation for developing a novel herbal-based oral care product that addresses the current challenges of antimicrobial resistance and treatment side effects.