Gc-Ms Instrument Analysis Test of Mint Leaf Extract Nanoemulsion ( Mentha Piperita L.) On the Reduction of NAFLD Scores and Necrosis Levels in Alloxan-Induced Hyperglycemic Wistar Rats and a picture of Histopathology Liver

Abstract

Study evaluated the effectiveness of mint leaf extract nanoemulsion (Mentha piperita L.) in reducing NAFLD scores and necrosis levels in alloxan-induced hyperglycemic Wistar rats. The method used included GC-MS instrument analysis to identify the active compounds in mint extract. Rats were divided into control and treatment groups, where the treatment group received mint extract nanoemulsion. The results showed that treatment with nanoemulsion significantly reduced NAFLD scores and liver necrosis compared to the control group. The content of active substances in mint leaf extract ( Mentha piperita L) through Gc Ms Analysis, namely Menthol : 45%, Menthone : 20%, Isomenthone : 10%, Limonene : 5%, Cineole (Eucalyptol) : 3%, Borneol : 2%, Carvone : 1% and Other Compounds : 14% (including fatty acids and other minor compounds). The results showed that administration of mint leaf nanoemulsion significantly reduced NAFLD scores, with an increasing effect at higher concentrations. The 5% group showed no NAFLD symptoms, indicating the most significant protective effect . Microscopic images of the liver of mice in treatment group 3 showed improved histological structure of the liver, so that it experienced improvement entering the score category 1 with no necrotic cells . This study concluded that mint leaf extract nanoemulsion could be a therapeutic alternative for liver disorders related to hyperglycemia.