Effectiveness Test of Chia Seed (Salvia Hispanica) Extract Nanoemulsion Preparation on Reducing Blood Glucose Levels (Kgd), Low Density Lipoprotein (LDL) Levels and Histopathological Features of the Liver of White Rats (Rattus Norvegicus) Wistar Strain In

Abstract

This lack of physical activity is often accompanied by excessive energy intake due to the wide variety of energy-rich foods, such as fast food. This study aims to test and analyze the effectiveness of chia seed (Salvia hispanica) extract nanoemulsion preparations on reducing blood cholesterol, low-density lipoprotein (LDL), and liver histopathology in male Wistar rats (Rattus norvegicus) induced by alloxan. Twenty-six rat samples were used in four groups (positive control, negative control, treatment 1, and 2), where the extract preparation in the treatment was chia seed extract nanoemulsion with concentrations of 10% and 20%. The results of histopathological observations showed that the negative control group experienced severe liver damage (score 3.16 ± 0.40), while the treatment group given chia seed extract nanoemulsion, especially the 20% concentration, showed improved liver structure with a lower damage score (1.5 ± 0.54). This indicates that chia seeds have the potential to protect liver tissue from damage.